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## SMRTSCAPE PacBio coverage estimatorSMRTSCAPE the % genome coverage and accuracy for different X coverage of a genome using PacBio sequencing, i.e. assuming a non-biased error distribution. Calculations use binomial/poisson distributions, assuming independence of sites. Accuracy is based on >50% reads covering a particular base having the correct call. Assuming random calls at the other positions, 25% of the wrong positions will be correct by chance. In reality, it will be even higher than this, assuming majority calls are used. Wrong calls will be split between three possible incorrect bases. Accuracy is therefore a conservative estimate. All calculations are based on © 2016 RJ Edwards. Contact: richard.edwards@unsw.edu.au. |